植物种子萌发调控信号研究获进展
来源:《自然—通讯》 时间:2016/10/25

 

 

记者从中科院华南植物园获悉,该园植物激素调控组助理研究员刘旭博士在侯兴亮研究员的指导下,发现NUCLEAR FACTOR Y-CNF-YC)家族基因在调控植物种子萌发中发挥重要作用。相关研究近日在线发表于《自然—通讯》

种子萌发是植物发育的一个重要阶段,决定着植物何时何地开启生长阶段。众多研究表明,种子萌发主要通过植物内源激素GAABA的相互制衡方式调节,然而这两种激素之间发生拮抗的具体分子机制迄今尚未阐释清楚。

研究人员发现,拟南芥NF-YC负调控GA介导种子萌发过程,在GA合成抑制剂(PAC)处理下,NF-YC同源基因多突变体的种子萌发率相比野生型显著提高,表现为PAC不敏感,而过表达NF-YC株系的种子对PAC超敏感。他们利用分子生物学手段找到了NF-YC的互作因子,即GA介导种子萌发关键抑制子DELLA蛋白RGL2

研究证实NF-YCRGL2在转录调控上具有相互依赖关系,二者共同结合到ABA信号关键因子ABI5基因启动子的CCAAT元件上促进其转录,以此来协同GAABA信号来介导种子萌发的调控。(来源:中国科学报 朱汉斌 周飞)

 

The NF-YC–RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis

 

Abstract  The antagonistic crosstalk between gibberellic acid (GA) and abscisic acid (ABA) plays a pivotal role in the modulation of seed germination. However, the molecular mechanism of such phytohormone interaction remains largely elusive. Here we show that three Arabidopsis NUCLEAR FACTOR-Y C (NF-YC) homologues NF-YC3, NF-YC4 and NF-YC9 redundantly modulate GA- and ABA-mediated seed germination. These NF-YCs interact with the DELLA protein RGL2, a key repressor of GA signalling. The NF-YC–RGL2 module targets ABI5, a gene encoding a core component of ABA signalling, via specific CCAAT elements and collectively regulates a set of GA- and ABA-responsive genes, thus controlling germination. These results suggest that the NF-YC–RGL2–ABI5 module integrates GA and ABA signalling pathways during seed germination.

 

原文链接:http://www.nature.com/articles/ncomms12768

 

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