人才队伍
吴中柳
  • 吴中柳
  • 电子邮件：wuzhl@cib.ac.cn
  • 职　　称：研究员
  • 学　　历：博士研究生
  • 通讯地址：四川省成都市天府新区群贤南街23号
  • 邮　　编：610213

个人简历

吴中柳研究员，女。2003年毕业于中国科学院上海有机化学研究所生物有机化学国家重点实验室，获理学博士学位；其后赴美国范德堡大学生物化学系从事博士后研究；2007年任中国科学院成都生物研究所应用与环境微生物研究中心研究员。入选2008年度四川省杰出青年学科带头人。目前以第一作者或者通讯作者在国际知名学术杂志上发表SCI研究论文60余篇，论文被引用超过1900次，H-index指数26 (根据ResearchGate统计)。已授权发明专利27项。  

研究方向

1、高效高选择性功能酶的筛选、表达和分子改造；  

2、新型多酶生物催化体系及化学-酶偶联体系的设计和构建； 

3、高值手性化合物的酶催化合成新方法和新工艺研究；  

4、生物催化法构建多样性的活性分子库和新型类药分子。


获奖及荣誉

2008，“四川省杰出青年学科带头人”

承担科研项目情况

国家自然科学基金（2008-2010，2011-2013，2014-2017，2016-2019，2021-2024） 

科技部科技重大专项，科技部中国越南政府间国际合作  

中国科学院知识创新工程重大项目专题   

中科院院部署项目，中科院STS项目  

四川省青年科技基金，四川省科技基金面上项目，企业横向委托项目等


代表论著

  1. Shi, M.-L.; Lin, R.-D.; Yu, Y.; Yao, Y.; Fan, X.-Y; Li, K.; Wu, Z.-L.; Yu, X.-Q.; Liu, Y.*; Wang. N.*. Efficient Stereoselective Synthesis of Aryl Lactones Using Engineered Ketoreductase ChKRED20 from Chryseobacterium sp. CA49. Organic Chemistry Frontiers. Org. Chem. Front., 2024, DOI: 10.1039/D3QO02076B.
  2. Lin, H.; Fang, S. Y.; Gao, H.; Qin, Z. Y.; Fan, D. L.; Li, N.; Wu, Z. L.; Chen, H. G., Mechanistic Insights into Enantiocontrolling of Styrene Monooxygenase-Catalyzed Epoxidation of Olefins. ACS Catal 2024, 14 (4), 2246-2251.
  3. Chen, Q.; Xiao, H.; Li, Z.-P.; Pei, X.-Q.; Yang, W.; Liu, Y.*; Wu, Z.-L.* Stereo-complementary epoxidation of 4-vinyl-2,3-dihydrobenzofuran using mutants of SeStyA with enhanced stability and enantioselectivity. Molecular Catalysis, 2023, 540: 113055.
  4. Zhu, B.-F.; Liu, Y.*; Pei, X.-Q.; Wu, Z.-L.* Characterization of Phenylalanine Ammonia Lyases from Lettuce (Lactuca sativa L.) as Robust Biocatalysts for the Production of D and L‑Amino Acids. J. Agric. Food Chem. 2023, 71 (6):2935-2942.
  5. Xing, Xiu; Liu, Yan; Lin, Ru-De; Zhang, Yang; Wu, Zhong-Liu; Yu, Xiao-Qi; Li, Kun; Wang, Na. Development of an integrated system for highly selective photoenzymatic synthesis of formic acid from CO2. Chemsuschem, 2023, 16(5):e202201956; DOI:10.1002/cssc.202201956.
  6. Liu, Y.; Chen, Q.; Zhu, B. F.; Pei, X.-Q.; Liu, Y.（刘艳）; Wu, Z. L.*. Sequence-guided stereo-enhancing and -inverting of (R)-styrene monooxygenases for highly enantioselective epoxidation. Molecular Catalysis 531 (2022) 112680
  7. Liu, Y.-C.; Wu, Z. -L.; Deska, J. Coding Synthetic Chemistry Strategies for Furan Valorization into Bacterial Designer Cells. ChemSusChem. 2023, 16(2): e202201790. DOI10.1002/cssc.202201790 .
  8. Lu, W.-F.; Yu, Y., Lin, R. D.; Yao, Y.; Liu, Y.; Wu, Z. L.; Liu, Y. *; Wang, N.* Enantioselective biosynthesis of (R)- -hydroxy sulfides via a one-pot approach with ChKRED20.Molecular Catalysis 531(2022)112655.
  9. Lin, H.*; Meng, Y.; Li, N.; Tang, Y.; Dong, S.; Wu, Z.; Xu, C.; Kazlauskas, R.*; Chen, H.*, Enzymatic Enantioselective anti-Markovnikov Hydration of Aryl Alkenes. Angew Chem Int Ed Engl 2022, 61 (32), e202206472.
  10. Yang, Y. J.; Pei, X. Q.; Liu, Y.; Wu, Z. L. *, Thermostabilizing ketoreductase ChKRED20 by consensus mutagenesis at dimeric interfaces. Enzyme Microb Technol 2022, 158, 110052.
  11. Xing, X.; Liu, Y.*; Shi, M.-L.; Li, K.; Fan, X.-Y.; Wu, Z.-L.; Wang, N.*; Yu, X.-Q. Preparation of chiral aryl alcohols: a controllable enzymatic strategy via light-driven NAD(P)H regeneration. New J. Chem. 2022, 46 (13), 6274-6282.
  12. Xiao, H.; Dong, S.; Liu, Y.; Pei, X.-Q.; Lin, H.*; Wu, Z.-L.* A new clade of styrene monooxygenases for (R)- selective epoxidation. Catalysis Science & Technology, 2021,11: 2195–2201.
  13. Liu, Y.*; Li, Z.-Y.; Guo, C.; Cui, C.; Lin, H.; Wu, Z.-L.* Enhancing the thermal stability of ketoreductase ChKRED12 using the FireProt web server. Process Biochemistry, 2021, 101:207-212
  14. Cui, C.; Lin, H. ; Pu, W. ;  Guo, C. ;  Liu, Y. ; Pei, X.-Q. ;  Wu, Z.-L.* Asymmetric Epoxidation and Sulfoxidation Catalyzed by a New Styrene Monooxygenase from Bradyrhizobium. Applied Biochemistry and Biotechnology, 2021, 193: 65-78.
  15. Li , C.; Pei, X.-Q.; Yi, D.; Li, T.-B.; Wu, Z.-L. Bioreductive dynamic kinetic resolution of ethyl 2-methoxy-3-oxo-3-phenylpropanoate. Catalysis Communications 135 (2020) 105865
  16. Cui, C.; Guo, C.; Lin, H.; Ding, Z.-Y.; Liu, Y.; Wu, Z.-L.* Functional characterization of an (R)-selective styrene monooxygenase from streptomyces sp. NRRL S-31. Enzyme and Microbial Technology, 2020, 132：109391
  17. Li, T.-B.; Zhao, F.-J.; Liu, Z.-C.; Jin, Y.; Liu, Y.; Pei, X.-Q.; Zhang, Z.-G.; Wang, G.-G*.; Wu, Z.-L.* Structure-guided engineering of ChKRED20 from Chryseobacterium sp. CA49 for asymmetric reduction of aryl ketoesters. Enzyme and Microbial Technology, 2019, 125: 29-36.
  18. Ren, Z.-Q.; Liu, Y.; Pei, X.-Q.; Wu, Z.-L.* Stereoselective bioreduction of ethyl 3-oxo-3-(2-thienyl) propanoate using the short-chain dehydrogenase/reductase ChKRED12. J. Microbiol. Biotechnol. (2019), 29(11), 1769–1776
  19. Pu, W.; Cui, C.; Guo, C.; Wu, Z.-L.* Characterization of two styrene monooxygenases from marine microbes. Enzyme and Microbial Technology, 2018, 112:29-34.
  20. Zhao, F.-J.; Jin, Y.; Liu, Z.-C.; Guo, C.; Li, T.-B.; Li, Z.-Y.; Wang G.-G.*; Wu, Z. -L*.Crystal structure and iterative saturation mutagenesis of ChKRED20 for expanded catalytic scope. Appl Microbiol Biotechnol. 2017, 101(23–24): 8395–8404.
  21. Guo, C.; Wu, Z.-L.* Construction and functional analysis of a whole-cell biocatalyst based on CYP108N7. Enzyme and Microbial Technology, 2017, 106：28-34.
  22. Liu, Y.-C.; Guo, C.; Liu, Y.; Wang, H.-B.; Wu, Z.-L.* Enzymatic cascades for the stereo-complementary epimerisation of in situ generated epoxy alcohols. Org. Biomol. Chem., 2017, 15, 2562-2568.
  23. Li, C.; Liu, Y.; Pei, X.-Q.; Wu, Z.-L.* Stereo-complementary bioreduction of saturated N-heterocyclic ketones, Process Biochem., 2017, 56：90-97.
  24. Zhao, F.-J.（#）; Liu, Y.（#）;Pei, X.-Q.;Guo, C.; Wu, Z.-L.* Single mutations of ketoreductase ChKRED20 enhance the bioreductive production of (1S)-2-chloro-1-(3,4-difluorophenyl) ethanol. Appl Microbiol Biotechnol, 2017,101（5）：1945-1952.
  25. Liu, Y.; Liu, Y.-C; Wu, Z.-L*. Asymmetric bio‑epoxidation catalyzed with the styrene monooxygenase from Pseudomonas sp. LQ26. Bioresour. Bioprocess. 2016, 100（8）:3567-3575.
  26. Xu, G.-P.; Wang, H.-B., Wu, Z.-L*. Efficient bioreductive production of (S)-N-Boc-3-hydroxypiperidine using ketoreductase ChKRED03. Process Biochem. 2016, 51: 881-885.
  27. Pei, X.-Q.; Xu, M.-Y.; Wu, Z.-L.* Two “classical” Old Yellow Enzymes from Chryseobacterium sp. CA49: broad substrate specificity of Chr-OYE1 and limited activity of Chr-OYE2. J. Mol. Catal. B: Enzym.2016, 123:91-99.
  28. Zhao, F.-J.; Pei, X.-Q.; Ren, Z.-Q.; Wu, Z.-L.* Rapid asymmetric reduction of ethyl 4-chloro-3-oxobutanoate using a thermostabilized mutant of ketoreductase ChKRED20. Appl Microbiol Biotechnol.2016. 100 (8): 3567-3575.
  29. Liu, Y.-C; Wu, Z.-L*. Switchable asymmetric bio-epoxidation of ,-unsaturated ketones.Chem. Commun. 2016, 52(6), 1158 – 1161.
  30. Li, J.-J.; Pei, X.-Q.; Zhang, S.-B.; Wu, Z.-L*. Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis. Process Biochem.2015, 50: 1783-1787.
  31. Li, J.-J.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L*. Removal of the free cysteine residue reduces irreversible thermal inactivation of Feruloyl Esterase: evidence from circular dichroism and fluorescence spectra. Acta Biochimica et Biophysica Sinica. 2015, 47(8):612-619.
  32. Ren, Z.-Q; Liu, Y.; Pei, X.-Q; Wang H.-B.; Wu, Z.-L*. Bioreductive production of enantiopure (S)-duloxetine intermediatescatalyzed with ketoreductase ChKRED15. J. Mol. Catal. B: Enzym.2015, 113,76–81.
  33. Liu, Y.-C; Liu, Y.; Wu, Z.-L*. Synthesis of enantiopure glycidol derivatives via a one-pot two-step enzymatic cascade. Org. Biomol. Chem., 2015, 13, 2146–2152
  34. Xu, M.-Y., Pei, X.-Q., Wu, Z.-L.* Identification and characterization of a novel "thermophilic-like" Old Yellow Enzyme from the genome of Chryseobacterium sp. CA49. J. Mol. Catal. B: Enzym.2014, 108, 64-71.
  35. Tang, T.-X.; Liu, Y; Wu, Z.-L.* Characterization of a robust anti-Prelog short-chain dehydrogenase/reductase ChKRED20 from Chryseobacterium sp. CA49. J. Mol. Catal. B: Enzym.2014, 105, 82-88.
  36. Liu, Y., Tang, T.-X., Pei, X.-Q., Zhang, C., Wu, Z.-L.* Identification of ketone reductase ChKRED20 from the genome of Chryseobacterium sp. CA49 for highly efficient anti-Prelog reduction of 3,5-bis(trifluoromethyl)acetophenone. J. Mol. Catal. B: Enz. 2014, 102(4), 1-8.
  37. Wang, H.-B., Pei, X.-Q.; Wu, Z.-L.* An enoate reductase Acrh-OYE4 from Achromobacter sp. JA81: Characterization and application in asymmetric bioreduction of C=C bonds. Appl. Microbiol. Biotechnol.2014, 98(2), 705-715.
  38. Yi, Z.-L.; Zhang, S.-B.*; Pei, X.-Q.; Wu, Z.-L.* Design of mutants for enhanced thermostability of b-glycosidase BglY from Thermus thermophilus. Bioresour. Technol.2013, 129, 629-633.
  39. Lin, H.; Tang, D.-F.; Ahmed, A. A. Q.; Liu, Y.; Wu, Z.-L.* Mutations at the putative active cavity of styrene monooxygenase: enhanced activity and reversed enantioselectivity. J. Biotechnol.2012, 161, 235-241.
  40. Zhang, S.-B.; Pei, X.-Q.; Wu, Z.-L.* Multiple amino acid substitutions significantly improve the thermostability of feruloyl esterase A from Aspergillus niger. Bioresour. Technol.2012, 117, 140-147.
  41. Liu, Y.-J.（#）; Pei, X.-Q.（#）; Lin, H.; Gai, P.; Liu, Y.-C.; Wu, Z.-L.* Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase. Appl. Microbiol. Biotechnol.2012, 95(3), 635-645.
  42. Zhang, S.-B.; Ma, X.-F.; Pei, X.-Q.; Liu, J.-Y.; Shao, H.-W.*, Wu, Z.-L.* A practical high-throughput screening system for feruloyl esterases: substrate design and evaluation. J. Mol. Catal. B: Enzym.2012, 74, 36-40.
  43. Lin, H.; Liu, J.-Y.; Wang, H.-B.; Ahmed, A. A. Q.; Wu, Z.-L.* Biocatalysis as an alternative for the production of chiral epoxides: a comparative review. J. Mol. Catal. B: Enzym.2011, 72(3-4), 77-89. (Invited review)
  44. Tang, C.-G.; Lin, H.; Zhang, C.; Liu, Z.-Q.; Yang, T.; Wu, Z.-L.* Highly enantioselective bioreduction of N-methyl-3-oxo-3- (thiophen-2-yl) propanamide for the production of (S)-duloxetine. Biotechnol. Lett. 2011, 33(7), 1435-1440.
  45. Lin, H.; Liu, Y.; Wu, Z.-L.* Highly diastereo- and enantio-selective epoxidation of secondary allylic alcohols catalyzed by styrene monooxygenase. Chem. Commun., 2011, 47, 2610-2612.
  46. Lin, H.; Liu, Y.; Wu, Z.-L.* Asymmetric epoxidation of styrene derivatives by styrene monooxygenase from Pseudomonas sp. LQ26: effects of - and -substituents. Tetrahedron: Asymmetry, 2011, 22, 134-137.
  47. Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum. Bioresour. Technol.2011, 102(3), 3636–3638
  48. Pei, X.-Q.; Yi, Z.-L.; Tang, C.-G.; Wu, Z.-L.* Three amino acid changes contribute markedly to the thermostability of -glucosidase BglC from Thermobifida fusca. Bioresour. Technol.2011, 102(3), 3337–3342.
  49. Qaed, A. A.; Lin, H.; Tang, D.-F.; Wu, Z.-L.* Rational design of styrene monooxygenase mutants with altered substrate preference. Biotechnol. Lett. 2011, 33, 611-616.
  50. Zhang, S.-B.; Wu, Z.-L.* Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus niger using the PoPMuSiC algorithm. Bioresour. Technol.2011, 102 (2), 2093–2096.
  51. Lin, H.; Qiao, J.; Liu, Y.; Wu, Z.-L.* Styrene monooxygenase from Pseudomonas sp. LQ26 catalyzes the asymmetric epoxidation of both conjugated and unconjugated alkenes. J. Mol. Catal. B: Enzym.2010, 67(3-4), 236-241.
  52. Zhang, Z.-G.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Improving the thermostability of Geobacillus stearothermophilus xylanase XT6 by directed evolution and site-directed mutagenesis. Bioresour. Technol.2010, 101(23), 9272-9278.
  53. Yi, Z.-L.; Wu, Z.-L.* Mutations from a family-shuffling-library reveal amino acid residues responsible for the thermostability of endoglucanase CelA from Clostridium thermocellumBiotechnol. Lett. 2010, 32(12), 1869-1875.
  54. Tang, Z., Salamanca-Pinzon, S.G., Wu, Z.L., Xiao, Y., Guengerich, F.P., Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function. Arch. Biochem. Biophys.2010, 494(1), 86-93.
  55. Wu, Z.-L.*; Qiao, J.; Zhang, Z.-G.; Guengerich, F.P.; Liu, Yan; Pei, X.-Q. Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression. Biotechnol. Lett. 2009, 31(10), 1589-1593.
  56. Zhang, Z.-G.; Liu, Y.; Guengerich, F.P.; Matse, J.H.; Chen J.; Wu, Z.-L.* Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries. J. Biotechnol.2009, 139(1), 12-18.



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