科研成果
| 论文编号: | DOI: 10.1080/10425170701606052 |
| 第一作者所在部门: | 应用与环境微生物研究中心 |
| 论文题目: | Cloning and characterization of farnesyl pyphosphate synthase gene from the ABA-producing fungi Botrytis cinerea |
| 作者: | HONG-YUAN DENG |
| 全部作者: | HONG-YUAN DENG,XIN-RONG MA,ZHI-DONG LI,HONG TAN |
| 论文出处: | |
| 刊物名称: | DNA Sequence |
| 年: | 2008 |
| 卷: | 19 |
| 期: | 3 |
| 页: | 313-318 |
| 联系作者: | 谭红 |
| 收录类别: | |
| 影响因子: | 0.606 |
| 摘要 : | Farnesyl pyphosphate synthase (FPPS) catalyzes the systhesis of farnesyl pyphosphate and appears to be a promising regulation site of Abscisic acid (ABA) biosynthesis pathway in fungi. Here we reported the isolation and characterization of Botrytis cinerea (FPPS) gene. The cloned FPPS gene carries an open reading frame of 1044-bp encoding a deduced protein of 347 amino acids with a molecular weight of 39.83 kDa, and the coding region is interrupted with a 63-bp intron. Comparison analysis showed that the deduced amino acids sequence share high similarity with other known FPPS gene. Southern blot revealed a single copy of FPPS gene in the genomic DNA. The result of transcription analysis indicated that the cloned FPPS gene expressed constitutively and was not induced in ABA accumulation phase. |
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