科研成果
| 论文编号: | |
| 第一作者所在部门: | 农业生物技术研究中心 |
| 论文题目: | Genetic Polymorphism of Low-Molecular-Weight Glutenin Genes at Glu-D3 Locus in a Wheat ( Triticum aestivum L. ) Variety, Chuannong 16 |
| 作者: | Zhuo Huang |
| 全部作者: | Zhuo Huang,Hai Long,Yu-Ming Wei,Ze-Hong Yan,You-Liang Zheng |
| 论文出处: | |
| 刊物名称: | Biotechnology |
| 年: | 2008 |
| 卷: | 7 |
| 期: | 3 |
| 页: | 544-550 |
| 联系作者: | You-Liang Zheng |
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| 摘要 : | In this study , a pair of degenerate PCR primer was designed base on the known LMW-GS genes , with which 4 district sequences were obtained from a weak gluten variety of wheat , Chuannong 16 , designated as LMWCN16-1 , LMWCN16-2 , LMWCN16-3 , and LMWCN16-4 , respectively . The total lengths of the 4 genes were 902 , 915 , 909 and 927 bp ,respectively . All these genes were predicted to be located at Glu-D3 locus and amount of DNA variations were found , including 97 nucleotide substitutions and several insertions and deletions (indels) . Sequence analysis indicated that the pairwise identities between the 4 genes were from 81.9% to 95.6% . LMWCN16-2 and LMWCN16-3 were more close related , while LMWCN16-4 was distinguished from LMWCN16-2 and LMWCN16-3 by a longer repetitive domain and the different position of the seventh cysteines involved in inter-molecular disulphide bond . These differences might result in different effects on the formation of the glutenin polymer and consequently the wheat quality . LMWCN16-1 is a putative pseudogene due to the five inframe stop codons and the frame shifting mutation in its C-terminal domain . The function loss of LMWCN16-1 might weaken the proportion of Glu-D3 in the total LMW-GS and bring negative influence on wheat quality . |
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