科研成果
| 论文编号: | DOI: 10.1134/S0026893308060186 |
| 第一作者所在部门: | 农业生物技术研究中心 |
| 论文题目: | Characterization and Expression Profiling of a Novel Cereal Cyst Nematode Resistance Gene Analog in Wheat |
| 作者: | 翟旭光 |
| 全部作者: | X. G. Zhai,T. Zhao,Y.H. Liu,H. Long,G.B. Deng,Z.F. Pan,M.Q. Yu |
| 论文出处: | |
| 刊物名称: | Molecular Biology |
| 年: | 2008 |
| 卷: | 42 |
| 期: | 6 |
| 页: | 960-965 |
| 联系作者: | 余懋群 |
| 收录类别: | |
| 影响因子: | 0.849 |
| 摘要 : | Based on the conserved regions of known resistance genes, an NBS–LRR-type CCN resistance gene analog was isolated from the CCN resistant E–10 near isogenic lines (NILs) of wheat, designated as CreZ(GenBank accession number: EU327996). It contained a complete ORF that was 2775 bp in length and encoded 924 amino acids. Sequence comparison indicated that it shared 92% nucleotide and 87% amino acid identity with those of the known CCN-resistance gene Cre3 and had similar characteristic conserved motifs to those in other established NBS–LRR disease resistance genes. The expression profiling of CreZ indicated that it was specifically expressed in the roots of resistant plants and real-time PCR analysis demonstrated that expression levels drastically increased when the plants were inoculated with cereal cyst nematodes. It could be inferred,then, that CreZ belongs to the NBS–LRR resistance gene family and is a candidate gene for potential resistance to the cereal cyst nematode. |
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