科研成果
| 论文编号: | DOI: 10.1002/rcm.3569 |
| 第一作者所在部门: | 天然产物研究中心 |
| 论文题目: | Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry |
| 作者: | 刘鑫 |
| 全部作者: | Xin Liu,Yan Zhou,Haibin Chen,Shulin Peng,Yucheng Gu,Lisheng Ding |
| 论文出处: | |
| 刊物名称: | RAPID COMMUNICATIONS IN MASS SPECTROMETRY |
| 年: | 2008 |
| 卷: | 22 |
| 期: | 12 |
| 页: | 1981-1992 |
| 联系作者: | 周燕，丁立生 |
| 收录类别: | |
| 影响因子: | 2.772 |
| 摘要 : | A rapid, simple electrospray ionization mass spectrometry (ESI-MS) sensitivity enhancement method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine hydrochloride (NH2OH·HCl) as a derivatization reagent. We use the oxime formed during the derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups. |
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