Enterobacter strains face limitations in scalable production. This study aimed to enhance fucose-containing exopolysaccharide (EPS) biosynthesis via UV mutagenesis and fermentation optimization. A high-yield mutant (Enterobacter sp. 915) was selected via UV irradiation, producing 55.93 % higher EPS yield (0.368 g/L) compared to the wild type. Response surface methodology (RSM) was used to optimize medium components and culture conditions. The optimal parameters were maltose (194.71 g/L), malt extract (108.18 g/L), and shaking speed (160 rpm), which increased EPS production to 2.304 g/L, a 74 % improvement. Structural analysis indicated the purified EPS (5.98 kDa) comprised fucose, galactose, glucose, and mannose in a ratio of 3.4:42.2:18.4:13, with α-glycosidic linkages and amorphous crystallinity. Thermal analysis showed stability up to 482.8 °C, indicating the potential for industrial processing. The EPS also demonstrated significant moisture retention (81 % RH: 24 h absorption rate >50 %) and antioxidant activities, including DPPH (34.33 %), hydroxyl (48.1 %), and ABTS+ (50.27 %) radical scavenging at 3.2 mg/mL. These properties were comparable to those of commercial fucose-containing EPS, demonstrating its potential as a natural antioxidant and humectant in food applications.