Sperm shape and size of five species of the genus Rhacophorus from China were investigated in the present study. Our results reveal the presence of two possible monophyletic lineages: the first is composed of R. chenfui, R.dugritei and R. omeimontis, has relatively small spermatozoa with a with a coiled head and a thin tail, while the second,composed of R. mutus and R. megacephalus, have spermatozoa that are longer with a straight head and filiform shape.