Cytogenetical comparison was made between high seed setting restores TP-4 and Dminghui63 with eminent maintainer line D64B of autotetraploid rice . The meiosis observation demonstrated that the genomes of our autotetraploid materials were all 2n=48 ,which was the same as those in mitosis observation . Low percentage of univalent and trivalent in diakinesis-metephase I (MI) of restoers TP-4 and Dminghui63 as well as maintainer line D46B of autoteraploid rice were observed . And the percentages of chromosome pairing were all over 99% , showing eminent cytological character . The frequency of TP-4 and Dminghui63 in diakinesis –metaphase I was 2.00/PMC (pollen mother cell) and 2.26/PMC , respectively . However , the frequency of D46B was 6.00/PMC ,significantly higher than those of TP-4 and Dminghui63 . It indicated that the maintainer D64B had better chromosome pairing capability in diakinesis-netaphase . While , the frequency of lagging chromosomes of maintainer D64B in anaphase I (AI) was 10.62%, significantly lower than that of TP-4 (19.44%) or Dminghui63 (23.14%) , and close to the level of diploid control (7.30%) . In telophase I (TI) , mantianer D64B exhibited lower frequency of microkernel and in telephase II (TII) the frequency of normal quartered microspore of maintainer D64B was not only higher than that of TP-4 or Dminghui63 but also than that of diploid control . The percentage of the cell observed chromosome lagging in AI and the percentage of abnormal cell in TI showed a greatly significantly positive correlation .That may demonstrate that chromosome seperation in AI and microkernel formation inTI are controlled by the same dominant single gene or the major gene of a QTL .